Fig. 1. Differential expression of NDRG2 gene and protein level in the cervical spinal cord of the wobbler mouse. (A) Expression levels of NDRG2 mRNA from presymptomatic to stationary phase of wild type (WT) and wobbler (WR) spinal cord were investigated by qPCR. Significant deregulation of NDRG2 was detected at all developmental stages. (B) Quantification of NDRG2 mRNA level in different regions of the spinal cord after laser micro dissection at p40. Significant deregulation was observed in all regions except the anterior column. For relative quantification of NDRG2 expression, the 2^-ΔΔCt method was conducted using the housekeeping gene GAPDH for normalization. Data are provided as means ± SEM. Data were tested for significance using Student's t-test. Significant differences are indicated by *p<0.1, **p<0.01, ****p<0.0001. n= 5-7. (C) Representative Western Blot image of NDRG2 expression levels in WT and WR mouse spinal cord at three developmental stages of the wobbler motor neuron disease. Two bands indicate two isoforms of NDRG2. Calnexin was used as control protein. (D) Quantitative analysis of NDRG2 protein expression level in the cervical spinal cord from presymptomatic to stationary phase of WT and WR spinal cord. A significant deregulation was found in developmental stages p20 and p40, while there is no significant deregulation at time point p0. Data are provided as means ± SEM. Data were tested for significance using Student's t-test. Significant differences are indicated by *p<0.1. n = 3-5.